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Detection and characterization of fumonisin mycotoxins as their methyl esters by liquid chromatography/particle‐beam mass spectrometry
Author(s) -
Young J. Christopher,
Lafontaine Pierre
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290070509
Subject(s) - chemistry , chromatography , mass spectrometry , trifluoroacetic acid , elution , chemical ionization , analytical chemistry (journal) , ionization , organic chemistry , ion
The diazomethane‐derived tetramethyl esters of fumonisins can be introduced readily into mass spectrometers using flow injection analysis or liquid chromatography (LC) via a particle‐beam interface to yield chemical ionization and electron‐ionization spectra. The esters of fumonisins B1, B2, and B3 were well resolved on a C18 reversed‐phase column when eluted with a gradient of water + methanol + acetonitrile in the presence of 0.05% trifluoroacetic acid. Enhancement of analyte transport through the particle‐beam interface to afford increased ion abundances was achieved by addition of carriers. Polyfunctional amino acid carriers, such as L ‐glutamic acid or L ‐serine, effected at least 100‐fold increases in sensitivity. In the LC mode, post‐column addition of aqueous L ‐serine was the most advantageous because background interference was limited to lower masses, and because of higher solubility, and ease of cleaning.