Premium
Analysis of cyclic nucleotide‐related enzymes by continuous‐flow fast‐atom bombardment mass spectrometry
Author(s) -
Langridge James I.,
Brenton A. Gareth,
Walton Terence J.,
Harris Frank M.,
Newton Russell P.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290070407
Subject(s) - chemistry , fast atom bombardment , mass spectrometry , substrate (aquarium) , continuous flow , nucleotide , enzyme , kinetics , phosphodiesterase , cyclic nucleotide phosphodiesterase , enzyme kinetics , chromatography , analytical chemistry (journal) , biochemistry , active site , oceanography , physics , quantum mechanics , mechanics , gene , geology
Continuous‐flow fast‐atom bombardment mass Spectrometry has been developed to directly monitor cyclic nucleotide (substrate) and its product levels from an on‐going phosphodiesterase reaction. Analysis of cAMP and cCMP phosphodiesterase incubates have been performed where the temporal evolution of the enzymic reaction is monitored and the effect of enzyme concentration upon the rate of reaction determined. Quantitative data on the enzyme kinetics have been obtained, in the form of Lineweaver‐Burke plots, that are shown to correlate well with well‐established radiometric methods.