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Charge‐remote fragmentation of bile acids derivatized with amino‐sulphonic acids
Author(s) -
Griffiths W. J.,
Zhang J.,
Sjövall J.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290070314
Subject(s) - chemistry , fragmentation (computing) , taurine , ion , dissociation (chemistry) , mass spectrum , conjugated system , amino acid , collision induced dissociation , spectral line , mass spectrometry , chromatography , stereochemistry , medicinal chemistry , organic chemistry , tandem mass spectrometry , biochemistry , polymer , computer science , operating system , physics , astronomy
Negative‐ion fast‐atom bombardment mass spectra of free and conjugated bile acids are dominated by [M H]− pseudomolecularions. Collision‐induced dissociations of these pseudomolecular ions give charge‐remote fragmentation patterns which are useful for structural identification. Taurine (2‐aminoethanesulrtionic acid)‐conjugated bile acids have a high cross‐section for charge‐remote fragmentation, and give the most informative and intense spectra among the naturally occurring bile acids. In the present study, collision‐induced dissociation spectra of bile acids derivatized with different aliphatic and aromatic aminosulphonates, and with 2‐amlnoethyl hydrogen sulphate have been compared. All show charge‐remote fragmentation patterns. Determination of the intensities of the fragment ions shows that the highest sensitivity is usually obtained with aromatic aininosulpho‐nate derivatives.