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In situ reduction suitable for matrix‐assisted laser desorption/ionization and liquid secondary ionization using tris(2‐carboxyethyl)phosphine
Author(s) -
Fischer Wolfgang H.,
Rivier Jean E.,
Craig A. Grey
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290070312
Subject(s) - chemistry , tris , in situ , phosphine , ionization , desorption , atmospheric pressure laser ionization , matrix (chemical analysis) , matrix assisted laser desorption electrospray ionization , analytical chemistry (journal) , matrix assisted laser desorption/ionization , chromatography , electron ionization , organic chemistry , ion , adsorption , biochemistry , catalysis
The suitability of the hydrochloride salt of tris(2‐carboxyethyl)phosphine (TCEP) for in situ reduction with matrix‐assisted laser desorption/ionization (MALDI) and liquid secondary ionization (LSI) mass spectrometry is evaluated. TCEP can be used to irreversibly reduce organic disulfldes to thiols in water and is active at an acidic pH. We found that TCEP was suitable for partial reduction of bovine insulin deposited on the target and mixed with either sinapinic acid in MALDI or glycerol and m ‐nitrobenzyl alcohol in LSIMS. When TCEP was added to insulin deposited on the target without prior mixing of sinapinic acid the in situ MALDI protocol resulted in almost complete reduction.