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Improved methodology for liquid chromatography/continuous flow secondary‐ion mass spectrometry: Quantitation of abscisic acid glucose ester using reaction monitoring
Author(s) -
Hogge Lawrence R.,
Balsevich John J.,
Olson Douglas J. H.,
Abrams Garth D.,
Jacques Suzanne L.
Publication year - 1993
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290070103
Subject(s) - chemistry , chromatography , detection limit , mass spectrometry , analyte , capillary action , analytical chemistry (journal) , selected ion monitoring , elution , selected reaction monitoring , ion suppression in liquid chromatography–mass spectrometry , liquid chromatography–mass spectrometry , matrix (chemical analysis) , tandem mass spectrometry , gas chromatography–mass spectrometry , materials science , composite material
Improvements in methodology for liquid chromatography/continuous flow secondary‐ion mass spectrometry (LC/CFSIMS) are described. Practical procedures have been developed for the preperation of packed fusedsilica capillary liquid chromatography columns and the coupling of these columns to the CFSIMS probe. A modified probe tip is described that significantly reduced ion beam instability arising from acceleration voltage discharge, changes in mobile phase composition during gradient elution, and uneven tip wettability. Interfering matrix background ions in LC/CFSIMS experiments are eliminated by reaction monitoring: the detection limit of the plant hormone metabolite, abscisic acid glucose ester, ABAGE is below 100 pg (235 fmol) with this technique. Reproducibility for quantitation is achieved by parallel reaction monitoring of the analyte and an added internal standard, ABAGE‐d 6 . The d 6 /d 0 ABAGE response ratio for the monitored transitions is linear over the range 100 pg to 10 ng of injected ABAGE.