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Determination on the microscale of plasmatic lactic acid as its t‐butyldimethylsilyl derivative by stable‐isotope dilution using capillary gas chrommatography/mass spectrometry
Author(s) -
Rocchiccioli F.,
Sanière M.
Publication year - 1992
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290061214
Subject(s) - chemistry , chromatography , lactic acid , mass spectrometry , isotope dilution , gas chromatography–mass spectrometry , microscale chemistry , derivative (finance) , capillary action , financial economics , genetics , mathematics education , mathematics , materials science , bacteria , economics , composite material , biology
A new sensitive and precise method for the determination of lactic acid in plasmatic microsamples (50 μL) has been developed. Lactic acid was directly extracted from plasma by ethyl acetate in acidic conditions, and analysed as its di‐t‐butyldimethylsilyl derivative by capillary gas chromatography/electron‐impact mass spectrometry (GC/MS). The internal pstandard was a previously synthesized deuterated compound, 3‐[ 2 H]‐(2R)‐lactic acid. The method gives good reproductibility and precision, the ovedrall standard deviation being better than 3%. The GC/MS assay was in good agreement with the enzymatic determination.