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Structure of underivatized branched oligosaccharides by negative‐ion fast‐atom bombardment mass spectrometry
Author(s) -
Garozzo D.,
Impallomeni G.,
Mantaudo G.,
Spina E.,
Daolio Sergio
Publication year - 1992
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290060903
Subject(s) - chemistry , fast atom bombardment , mass spectrum , trisaccharide , mass spectrometry , ion , molecule , monosaccharide , fucose , analytical chemistry (journal) , stereochemistry , chromatography , organic chemistry , galactose
We report here a study by negative‐ion fast‐atom bombardment (FAB) mass spectromatry of branched oligosacharides containing a 3‐O glyesidie linkage. Our result show that discrimination of the monosaccharide unit linked in the 3‐O position is made possible by the analysis of the negative‐ion FAB mass spectra. In the mass spectrum of A‐trisaccharide (α‐Fuc1‐2‐(α‐GalNAc1‐3)‐Gal) the peak at m / z 307 can be explained as elimination of a neutral molecule of GalNAc(221 u) from the molecular ion. Also in the mass spectrum of 3‐fucosyllaactose (α‐Fuc1‐3‐(β‐Gall‐4)‐Glc) an intense peak at m / z 323, corresponding to the elimination of the fucose linked at the 3 position of glucose is present. To investigate further the fragmentations of the 3‐O‐linked sugars, the negative‐ion FAB mass spectra of disaccharides have also been studied.