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Determination of 15 N enrichment of taurine in cat urine by high resolution fast‐atom bombardment mass spectrometry
Author(s) -
Stämpfli Andress A.,
Ballèvre Olivier,
Fay Laurent B.
Publication year - 1992
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290060902
Subject(s) - taurine , chemistry , chromatography , mass spectrometry , urine , fast atom bombardment , resolution (logic) , ion , hydrolysis , high resolution , analytical chemistry (journal) , amino acid , biochemistry , remote sensing , organic chemistry , artificial intelligence , computer science , geology
A method is described for measuring the stable isotopic enrichment of taurine in cat urine samples by high resolution fast‐arom bombardment mass spectrometry, after 15 N labelled taurine was given to cats for the purpose of investigation taurine metabolism. The 15 N enrichemnt of taurine was measurd after hydrolysis and purification of taurine by anion/cation exchange chromatography. The isotopic ratio of taurine was detemined by measuring the [M+H] + ion peaks in the spectra of the unlabelled and labelled compounds under multiple ion scan conditions. The overall standard deviation of the measurement is beter than 4%. This method requires no derivation and uses only 500 μL of urine samples.