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High‐performance anion‐exchange chromatography/thermospray mass spectrometry in the analysis of oligosaccharides
Author(s) -
Niessen W. M. A.,
van der Hoeven R. A. M.,
van der Greef J.,
Schols H. A.,
LucasLokhorst G.,
Voragen A. G. J.,
Bruggink C.
Publication year - 1992
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290060714
Subject(s) - chemistry , thermospray , chromatography , mass spectrometry , ion chromatography , oligomer , ion exchange , polysaccharide , oligosaccharide , ion , tandem mass spectrometry , selected reaction monitoring , organic chemistry
The on‐line coupling of high‐performance anion‐exchange chromatography mass spectrometry is described. The system is applied to the analysis of neutral and acidic oligosaccharides obtained by (enzymic) degradation of plant‐cell‐wall polysaccharides. The features, potential and limitations of the method are discussed and exemplified with the analysis of some glucuronoxylan and xyloglycan digests. The system allows rapid molecular‐mass determination of components in oligomer mixtures with 1–10 μg injected amounts.