Premium
Matrix‐assisted laser desorption mass spectrometry of oligodeoxythmidylic acids
Author(s) -
HuthFehre T.,
Gosine J. N.,
Wu K. J.,
Becker C. H.
Publication year - 1992
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290060312
Subject(s) - chemistry , mass spectrometry , adduct , fragmentation (computing) , desorption , oligonucleotide , matrix (chemical analysis) , resolution (logic) , ferulic acid , analytical chemistry (journal) , chromatography , organic chemistry , dna , biochemistry , adsorption , artificial intelligence , computer science , operating system
Abstract Ferulic acid, sinapinic acid and 2,5‐dihydroxybendoic acid (DHBA) have been tested as matrix materials for matrix‐asserted laser desorption of the pure oligonucleotide pd(T) 12 and a mixture of oligonucleotides pd(T) 12 through pd(T) 18 using pulsed 337 nm radiation combined with reflecting time‐of‐flight mass spectrometry. The three matrix materials are compared with respect to obtainable mass resolution, degree of fragmentation, and adduct formation, as well as the highest mass resolution.