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Studies on the metabolism of omeprazole in the rat using liquid chromatography/ionspray mass spectrometry and the isotope cluster technique with [ 34 S] omeprazole
Author(s) -
Weidof Lars,
Covey Thomas R.
Publication year - 1992
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290060308
Subject(s) - chemistry , chromatography , mass spectrometry , omeprazole , metabolite , electrospray ionization , isotope dilution , sample preparation , electrospray , analytical chemistry (journal) , medicine , biochemistry
This paper describes the application of the ionspray (pneumatically‐assisted electrospray) interface for liquid chromatography (LC) and atmospheric‐pressure ionization mass spectrometry (API MS) to samples obtained in a study on the metabolism of omeprazole. In this study [ 34 S]omeprazole was utilized for the stable isotope cluster technique. Over forty metabolites in a sample of partially purified rat urine were resolved by gradient elution LC with ionspray API MS detection, and each of them produced molecular ion 1:1 clusters (MH + and [MH+2] + ). The Chromatographic fidelty of the total‐ion current (TIC) was excellent. The endogenous matrix of the sample was quite low, allowing a background‐substracted averaged mass spectrum of the entire TIC trace to produce a ‘metabolite mass profile’ depicting all the molecular ion 1:1 clusters in the sample. From this mass profile, it was possible to obtain direct information concerning oxygenation an conjugation reactions of the parent compound.