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Accurate mass measurement of proteins using electrospray ionization on a magnetic sector instrument
Author(s) -
McEwen C. N.,
Larsen R. S.
Publication year - 1992
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290060304
Subject(s) - chemistry , mass spectrometry , electrospray ionization , calibration , analytical chemistry (journal) , resolution (logic) , electrospray , ionization , chromatography , ion , physics , organic chemistry , quantum mechanics , artificial intelligence , computer science
Peptides and proteins with molecular weights of ca 20 000 have been mass measured with accuracies of ±0.5 Da using electrospray ionization interfaced to a double‐focusing magnetic sector mass spectrometer. Factors affecting mass measurement accuracy such as resolution, peak profiles, calibration, data processing, and mass calculations are discussed.