Premium
Analysis of saxitixin in urine by continuous‐flow fast‐atom bombardment mass spectromety
Author(s) -
Mirocha Chester J.,
Cheong Wonjo,
Mirza Urooj,
Kim Young Bae
Publication year - 1992
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290060211
Subject(s) - chemistry , fast atom bombardment , chromatography , analytical chemistry (journal) , matrix (chemical analysis) , polyethylene glycol , mass spectrometry , extraction (chemistry) , volumetric flow rate , organic chemistry , physics , quantum mechanics
An improved method of saxitoxin analysis in urine using continuous‐flow fast‐atom bombardment mass spectrometry was developed. Parameters studied were matrix composition, matrix flow, temperature of probe tip, probe‐tip design and sample extraxtion. Optimal detection was obtained using the following matrix composition: 5% glycerol, 0.5% acetic acid, 0.025% sodium dodecylsulfate, 0.1% polyethylene glycol (PEG) 400 and 0.5% PEG 300; probe‐tip temperature: (∼55°C); flow rate: 5 or 8μL per min.; probe tip: Olson‐Hogge design. The STX standard was detected at 200pg with signal‐to‐noise ratio of 11. The percent recovery of saxitoxin from human urine after clean‐up on a weak cation exchange column was 75%.