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Ionspray mass spectrometry of marine toxins. IV. Determination of diarrhetic shellfish poisoning toxins in mussel tissue by liquid chromatography/mass spectrometry
Author(s) -
Pleasance Stephen,
Quilliam Michael A.,
Marr Julie C.
Publication year - 1992
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290060210
Subject(s) - chromatography , diarrhetic shellfish poisoning , okadaic acid , mussel , chemistry , marine toxin , mytilus , detection limit , mass spectrometry , shellfish poisoning , shellfish , saxitoxin , toxin , fish <actinopterygii> , fishery , biology , aquatic animal , biochemistry , phosphorylation , phosphatase
An Improved liquid chromatographic/mass spectrometric (LC/MS) method utilizing gradient elution and ion‐spray ionization is described for the sensitive determination of okadaic acid and dinophysistoxin‐1, the principal toxins implicated in cases of diarrhetic shellfish poisoning. The method was used to confirm the presence of both toxins, together with a recently identified isomer of okadaic acid, dinophysistoxin‐2, in various samples of cultivated blue mussels ( Mytilus edulis ) from Canadian and European Waters. The method provided a mass detection limit of 0.4 ng for each toxin, thus allowing detection of 40 ng per g of whole mussel tissue (or approximately 10 ng/g if only the digestive glands were used in the assay). Quantitative results obtained by LC/MS were in good agreement with those obtained by derivatization and high‐performance liquid chromatography with fluorescence detection.