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Peptide sequencing by matrix‐assisted laser‐desorption mass spectrometry
Author(s) -
Spengler Bernhard,
Kirsch Dieter,
Kaufmann Raimund,
Jaeger Ernst
Publication year - 1992
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290060207
Subject(s) - reflectron , chemistry , mass spectrometry , metastability , ion , analytical chemistry (journal) , kinetic energy , protein mass spectrometry , sample preparation in mass spectrometry , time of flight mass spectrometry , chromatography , tandem mass spectrometry , ionization , electrospray ionization , organic chemistry , physics , quantum mechanics
A novel method of peptide sequencing by mass spectrometry is described. Metastable decay of laser‐desorbedions, taking place in the first field‐free drift region of a reflectron time‐of‐flight mass spectrometer, has been monitored to get structural information from larger peptides. Fragment ions from metastable decay are mass analysed by adjusting the potentiaals of the ion reflectron according to the kinetic energies of the ions. The features of the technique and its significance for future applications are outlined.