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Development of a condensation technique for thin‐layer chromatography/fast‐atom bombardment mass spectrometry of non‐visible compounds
Author(s) -
Oka Hisao,
Ikai Yoshitomo,
Kondo Fumio,
Kawamura Norihisa,
Hayakawa Junko,
Masuda Katsuyoshi,
Harada KenIchi,
Suzuki Makoto
Publication year - 1992
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290060204
Subject(s) - chemistry , mass spectrometry , fast atom bombardment , analytical chemistry (journal) , chromophore , chromatography , mass spectrum , condensation , thin layer chromatography , reproducibility , ion , organic chemistry , physics , thermodynamics
Abstract In Order to prevent the diffusion of sample when a matrix is deposited on the sample spot on a thin‐layer chromatography (TLC) plate and to obtain constructively high sensitivity in mass spectrometry, we developed a conedensation technique including ‘trapezoid technique’ for TLC/fast‐atom bombardment mass spectrometry (FABMS) on both silica gel and C18 plates. For colored samples and colorless samples having a chromophore, the condensation technique previously in use, that condenses the sample towards the center of the spot in a line, using methoanol, was effective. The trapezoid condensation technique has been newly developed for colorless compounds having no chromophore. The mass spectra obtained using this technique clerly showed molecular ion species at relatively lower concentrations of samples, and the technoique can improve the detection limits of molecular ion species in TLC/FABMS by 3‐100 times with good reproducibility.