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The use of 4‐hydroxybenzenesulfonic acid as a reduction‐inhibiting matrix in liquid secondary‐ion mass spectrometry
Author(s) -
Visentini J.,
Nguyen P. M.,
Bertrand Michel J.
Publication year - 1991
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290051204
Subject(s) - chemistry , fast atom bombardment , mass spectrometry , matrix (chemical analysis) , chromatography , fragmentation (computing) , ion , mass spectrum , organic chemistry , computer science , operating system
Abstract The use 4‐hydroxybenzenesulfonic acid as reduction‐inhibiting and general purpose matrix in fast‐atom bombardment liquid secondary‐ion mass spectrometry (FAB/LSIMS) is discussed. Results obtained in a comparative study using the disulfide‐bridge containing peptide deaminoarginine‐vasopressin and several commonly used matrices indicate that the reduction‐inhibitaing efficiency of 4‐hydroxybenzenesulfonic acid is comparable to that of 3‐nitrobenzyl alcohol while recuction is found to be important in matrices such as thioglycerol and glycerold. Experiments conducted on peptides such as met‐enkephalin and bradykinin, nucleosides and other polar compounds reveal that 4‐hydroxybenzenesulfonic acid is a good solvent and has physical properties which are similar to those of glycerol. This new matrix material is a radical scavenger, surface active, allows persistent secondary‐ion beams and produces a low background signal. The matrix, 4‐hydroxy‐benzenesulfonic acid, generally yields high quality mass spectra which compare well with those obtained in either glycerol or 3‐nitrobenzyl alcohol.