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Differentiation of type 1 and type 2 chain linkages of native glycosphingolipids by positive‐ion fast‐atom bombardment mass spectrometry with collision‐induced dissociation and linked scanning
Author(s) -
Salyan Mary Ellen K.,
Stroud Mark R.,
Baillie T. A.,
Levery Steven B.
Publication year - 1991
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290051007
Subject(s) - chemistry , fast atom bombardment , mass spectrometry , glycoconjugate , dissociation (chemistry) , collision induced dissociation , ion , tetrasaccharide , stereochemistry , tandem mass spectrometry , analytical chemistry (journal) , chromatography , organic chemistry , biochemistry , polysaccharide
Two underivatized glycosphingolipids, Le b and Le y , isomeric in carbohydrate structure (Fucα1 → 2Galβ1 → 3[Fucα1 → 4]GlcNAcβ1 → 3Glcβ1 → 4Glcβ1 → 1Cer and Fucα1 → 2Galβ1 → 4[Fucα1 → 3]GlcNAcβ1 → 3Galβ1 → 4Glcβ1 → 1Cer, respectively), were analyzed by positive‐ion fast‐atom bombardment (FAB) mass spectrometry with high energy collision‐induced dissociation (CID) and linked scanning. The two isomers were distinguishable by the abundance of product ions derived from the non‐reducing terminal tetrasaccharide fragment via sequential β‐eliminations of vivinaliy linked saccharide residues. Following earlier studies from other laboratories, which have dealt primarily with positive‐ion FAB‐CID mass spectrometry of simple model oligosaccharides, these results exemplify the practical application of two‐sector methodology to underivatized complex glycoconjugates commonly encountered in the biomedical field.