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Determination of the glycosidic linkage in peracetylated disaccharides comprised of d‐glucopyranose units by use ofr desorption electron‐ionization mass spectrometry
Author(s) -
Peltier John M.,
MacLean David B.,
Szarek Walter A.
Publication year - 1991
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290051005
Subject(s) - oxonium ion , chemistry , glycosidic bond , moiety , ion , electron ionization , mass spectrometry , mass spectrum , desorption , ionization , polyatomic ion , analytical chemistry (journal) , stereochemistry , organic chemistry , chromatography , adsorption , enzyme
An oxonium ion at m/z 317 is present in the desorption electron ionization and ammonia desorption chemical ionization mass spectra of peracetylated disaccharides, comprised of glucopyranose units linked (1 → 2), (1 → 3), (1 → 4) and (1 → 6), but is absent in the spectra of the (1 → 1)‐linked isomer. The ion aat m/z 317, which is derived from the reducing moiety, has an O‐formyl group at the position of linkage to the non‐reducing moiety, and O‐acetyl groups at each of the remaining positions. The iosmeric monoformyl, triacetyl oxonium ions (at m/z 317), derived from the (1 → 2)‐, (1 → 3)‐, (1 → 4)‐ and (1 → 6)‐linked disaccharides, give distinctly different mass‐analysed ion kinetic energy spectra, thereby enabling the linkage position to be assigned unambiguously.