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Massive cluster impact mass spectrometry: A new desorption method for the analysis of large biomolecules
Author(s) -
Mahoney J. F.,
Perel J.,
Ruatta S. A.,
Martino P. A.,
Husain S.,
Cook Kelsey,
Lee T. D.
Publication year - 1991
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290051004
Subject(s) - chemistry , cluster (spacecraft) , mass spectrometry , biomolecule , desorption , chromatography , analytical chemistry (journal) , adsorption , biochemistry , computer science , programming language
A new ion desorption method is described that utilizes a primary beam of massive, multiply charged cluster ions to generate secondary ions of peptides in a glycerol matrix. The massive cluster ion beam is generated via electrohydrodynamic emission using a 1.5 M solution of ammonium acetate in 30% aqueous glycerol. Negaitve ion spectra of peptides obtained using this technique show greatly decreased relative intensities for fragment ions and ‘chemical noise’ background when compared to spectra obtained using a xenon atom primary beam. The near absence of fragmets derived from radiation damage to the sample solution is attributed to the impact of primary particles with energies less than 1 eV/nucleon.