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Analysis of bovine β‐casein tryptic digest by continuous‐flow fast‐atom bombardment mass spectrometry
Author(s) -
Jones D. S.,
Heerma W.,
van Wassenaar P. D.,
Haverkamp J.
Publication year - 1991
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290050410
Subject(s) - chemistry , fast atom bombardment , mass spectrometry , chromatography , fragmentation (computing) , casein , amino acid , analytical chemistry (journal) , biochemistry , computer science , operating system
Liquid chromatography/fast‐atom bombardment mass spectrometry was used to partially confirm the amino‐acid sequence of the protein, β‐casein. The study demonstrates that the technique is capable of the rapid and accurate identification of peptide fragments from tryptic digests and that chromatograrphic integrity is maintained during the analysis. The power of the technique derives from the ability to determine both the retention time and the molecular weight of the eluting components. Each of the components yields a prominent pseudo‐molecular ion (MH + ), the majority exhibiting sufficient fragmentation to confirm their structure unambiguously.