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Matrix/sample interactions in ultraviolet laser‐desorption of proteins
Author(s) -
Doktycz Stephen J.,
Savickas Philip J.,
Krueger Duane A.
Publication year - 1991
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290050402
Subject(s) - chemistry , ultraviolet , desorption , matrix (chemical analysis) , sample (material) , laser , soft laser desorption , chromatography , matrix assisted laser desorption/ionization , environmental chemistry , analytical chemistry (journal) , optoelectronics , organic chemistry , optics , adsorption , physics
Examination, by optical microscopy, of the matrix/analyte morphology of a sample propared from a droplet in matrix‐assisted UV laser‐desorption mass spectrometry (MUVLDMS) reveals that the matrix crystallizes to a large extent, thus altering the concentration of the material in regions on the surface from that of the bulk applied solution. Microscopy reveals that vanillic acid and to a lesser extent nicotinic acid matrices form prominent crystalline rings around the dried sample spot while sinapic acid forms a more uniform dispersion of the crystallized matrix. Insulin analyte investigated in this report tendss to form a thin isotropic phase in the center of the drops. The first reported use of a KrF excimer laser (249 nm) in MUVLDMS is also discussed.