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Matrix‐assisted laser desorption of large biomolecules with a TEA‐CO 2 ‐laser
Author(s) -
Overberg A.,
Karas M.,
Hillenkamp F.,
Cotter R. J.
Publication year - 1991
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290050308
Subject(s) - chemistry , biomolecule , laser , desorption , matrix (chemical analysis) , analytical chemistry (journal) , nanotechnology , chromatography , optics , organic chemistry , adsorption , biochemistry , physics , materials science
Abstract Matrix‐assisted desorption/ionization of large biomolecules with a TEA‐CO 2 laser with a warelength of 10.6 μm is demonstrated. TEA‐CO 2 LDI mass spectra are almost identical to those obtained with the Er‐YAG (2.94 μm) infra‐red (IR) laser and very similar to UV‐LDI spectra. Most of the matrices usable for Er‐YAG IR‐LDI have been tested at 10.6 μm with success, and the main features of IR‐LDI e.g., the tendency towards the formation of higher chargers is shown.