Premium
Location of unsaturated positions in phosphatidyl cholines by consecutive‐reaction monitoring
Author(s) -
Bryant Duncan K.,
Orlando Ron,
Boyd R. K.
Publication year - 1991
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290050307
Subject(s) - chemistry , tandem , ion , tandem mass spectrometry , mass spectrometry , chromatography , carboxylate , organic chemistry , materials science , composite material
Consecutive‐reaction monitoring (MS 3 ) of negative ions has been shown to be useful in locating double bonds in the fatty acid ester groups of individual phosphatidyl cholines present in complex mixtures. This information is derived from charge‐remote fragmentations of carboxylate anions produced by collisional activation of the [MCH 3 ] − precursor ions from phosphatidyl cholines. The added selectivety of this technique increases the sensitivity, relative to that of tandem mass spectrometry (MS 2 ), such that only 3 pmol are needed to perform this analysis.