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Phase‐system switching in liquid chromatography/continuous‐flow fast‐atom bombardment mass spectrometry
Author(s) -
Kokkonen P. S.,
Niessen W. M. A.,
Tjaden U. R.,
van der Greef J.
Publication year - 1991
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290050106
Subject(s) - chemistry , chromatography , mass spectrometry , high performance liquid chromatography , volumetric flow rate , analytical chemistry (journal) , fast atom bombardment , analyte , splitter , physics , geometry , mathematics , quantum mechanics
A new, attractive application of the phase‐system switching (PSS) technique is demonstrated. The PSS system is used in combination with liquid chromatography coupled to continuous‐flow fast‐atom bombardment mass spectrometry (LC/CF‐FABMS) to achieve flow‐rate reduction from 1.0 mL/min in the high‐performace liquid chromatography (HPLC) system to 15μL/min going into the mass spectrometer. In PSS, a compound of interest, in this case erythromycin 2′‐ethylsuccinate, is enriched after the analytical separation, on a short trapping column, from which it is desorbed subsequently to the LC/MS interface. Optimal mobile phases and flow‐rates can be used for the HPLC separation as well as for the mass spectrometric detection. Desorption of the analyte is achieved either by using a flow‐rate of 15 μL/min without a splitter or a flow‐rate of 230 μL/min with a splitter. Compared to the LC/CF‐FAB MS methods using a conventional bore HPLC column and a splitter, improved detection limits for the model compound were achieved using the PSS method.