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Characterization of cyclodextrins using ion‐evaporation atmospheric‐pressure ionization tandem mass spectrometry
Author(s) -
Huang Eric C.,
Henion Jack D.
Publication year - 1990
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290041103
Subject(s) - chemistry , tandem mass spectrometry , mass spectrometry , collision induced dissociation , electrospray ionization , ion source , protonation , analytical chemistry (journal) , ion , chromatography , dissociation (chemistry) , selected reaction monitoring , protein mass spectrometry , organic chemistry
A tandem mass spectrometer equipped with the pneumatically‐assisted electrospray (ion spray) liquid chromatography/mass spectrometry interface and an atmospheric‐pressure ionization source was used for characterization of underivatized cyclic oligosaccharides. Protonated singly charged molecular ions were generated via ion‐evaporation mechanism(s). The formation of doubly charged molecular ions from the model compounds appears to be related to the channel diameter of these cyclic oligosaccharides. Tandem mass spectrometry (MS/MS) was used to study collision‐induced dissociation of the protonated molecule and showed exclusive cleavage of the glycosidic bond. The molecular weights of these oligosaccharides as well as the number of sugar units were confirmed by MS/MS. The potential application of MS/MS for target compound analysis was demonstrated by the parent‐ion scan method with a synthetic mixture.