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The use of tandem mass spectrometry for the differentiation of bile acid isomers and for the identification of bile acids in biological extracts
Author(s) -
Eckers Christine,
New Anthony P.,
East Philip B.,
Haskins Neville J.
Publication year - 1990
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290041024
Subject(s) - chemistry , mass spectrometry , tandem mass spectrometry , chromatography , tandem , fast atom bombardment , bile acid , ion , mass spectrum , triple quadrupole mass spectrometer , protein mass spectrometry , liquid chromatography–mass spectrometry , hybrid mass spectrometer , selected reaction monitoring , analytical chemistry (journal) , organic chemistry , biochemistry , materials science , composite material
Tandem mass spectrometric (MS/MS) techniques have been widely used for the differentiation of isomeric compounds, since their spectra may show differences sufficient to distinguish between them. There are several different ways by which the MS/MS data can be obtained depending on the energies of the ions and the collisions. In this paper MS/MS spectra have been obtained for a group of isomeric bile acids using: 1, low‐energy ions and low‐energy collisions in a triple quadrupole mass spectrometer by liquid chromatography/MS/MS; 2, high‐energy ions and low‐energy collisions in a hybrid mass spectrometer by fast‐atom bombardment MS/MS. Liquid chromatography combined with tandem mass spectrometry (LC/MS/MS) has also been used to identify the bile acids present in biological matrices such as bile extracts.