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On‐line combination of supercritical‐fluid chromatography with fast‐atom bombardment mass spectrometry
Author(s) -
Matsuura K.,
Takeuchi M.,
Nojima K.,
Kobayashi T.,
Saito T.
Publication year - 1990
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290041007
Subject(s) - chemistry , supercritical fluid chromatography , chromatography , mass spectrometry , fast atom bombardment , frit , analytical chemistry (journal) , ethylene glycol , supercritical fluid , matrix (chemical analysis) , high performance liquid chromatography , organic chemistry , materials science , metallurgy
Studies on combined supercritical‐fluid chromatography (SFC) using a packed column, with fast‐atom bombardment mass spectrometry (FAB‐MS) are described. The interfacing was successfully carried out by simply coupling a frit interface between the liquid chromatograph (LC) and the mass spectrometer. The interface was equipped with a pneumatic splitter and post‐column addition device delivering FAB matrix material to a release valve which maintained a constant back‐pressure at the column outlet. Using a 1% glycerol solution as a matrix compound in the release valve, the SFC/FAB‐MS combination operates as well as a normal LC/MS instrument. The performance was demonstrated with a mixture of α‐, β‐ and γ‐tocopherol and Triton X‐100 (poly(ethylene glycol) p ‐isooctylphenyl ether).