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Matrix‐assisted laser desorption of peptides in transmission geometry
Author(s) -
Vertes Akos,
Balazs Laszlo,
Gijbels Renaat
Publication year - 1990
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290040709
Subject(s) - chemistry , desorption , matrix (chemical analysis) , bombesin , substrate (aquarium) , ion , mass spectrum , adduct , spectral line , molecule , laser , metal ions in aqueous solution , analytical chemistry (journal) , chromatography , organic chemistry , neuropeptide , biochemistry , optics , oceanography , receptor , physics , adsorption , astronomy , geology
The possibility of performing matrix‐assisted laser desorption experiments in transmission geometry is demonstrated for two neuropeptides (substance P and bombesin), for six analogs of the melanocyte‐stimulating hormone (MSH) core and for coliagenase enzyme substrates. Positive‐ and negative‐ion spectra of several peptides are produced without the presence of a metallic substrate. Cationized quasi‐molecular ions are abundant in the positive spectra. Peak broadening in the high‐mass range can be the consequence of overlapping molecular and adduct ions. The presence of synthesis by‐products can be identified readily from the spectra. Ultimately, picogram detection limits are possible for important bioactive peptides and other large molecules. Because of the clearly demonstrated matrix‐assisted laser ionization in a homogeneous environment, metal substrate participation in the volatilization mechanism seems less likely.