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Effect of different target gases on low‐energy collision‐activated dissociation of peptides
Author(s) -
Naylor Stephen,
Lamb John H.
Publication year - 1990
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290040706
Subject(s) - chemistry , fragmentation (computing) , ion , dissociation (chemistry) , protonation , mass spectrometry , collision induced dissociation , collision , proton affinity , mass spectrum , atomic physics , ionization , nitrogen , bond dissociation energy , analytical chemistry (journal) , tandem mass spectrometry , organic chemistry , chromatography , physics , computer security , computer science , operating system
Experimental variables affecting the daughter‐ion spectra of a series of protonated peptides [MH] + , produced by fast‐atom bombardment ionization, using a low energy (0–450 eV) quadruple collision cell are investigated. The parameters studied include target gas pressure, collision energy, cross‐sectional area and acidity of the target gas. The results show that low‐mass immonium ions are preferentially formed both at high collision energies (> 200 eV) and at target gas pressures > 10 −6 mBar (where multiple collisions occur in the gas cell). Positive fragment ion abundance is maximized when acidic gases are used as the target gases, and this is rationalized on the basis of a proton‐transfer reaction from the target gas to the amide nitrogen of the peptide bond promoting fragmentation.