Premium
Quantitative analysis of 1‐stearoyl‐2‐oleoyl‐ sn ‐glycero‐3‐phosphoserine by negative‐ion fast‐atom bombardment mass spectrometry
Author(s) -
Chen Su,
Kirschner Günther,
Benefenati Emilio
Publication year - 1990
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290040609
Subject(s) - chemistry , phosphoserine , calibration curve , mass spectrometry , ion , fast atom bombardment , analytical chemistry (journal) , range (aeronautics) , chromatography , detection limit , biochemistry , serine , organic chemistry , enzyme , materials science , composite material
Negative‐ion fast‐atom bombardment mass spectrometry was evaluated as a means for quantitative analysis of individual molecular species of glycerophosphatidylserine (GPS). With 200 ng of 1,2‐dipalmitoyl‐ sn ‐glycero‐3‐phosphoserine (GPS di 16:0) as an internal standard, the calibration curve for 1‐stearoyl‐2‐oleoyl‐ sn ‐glycero‐3‐phosphoserine (GPS 18:0–18:1) was linear over a range of 50–1000 ng (signal‐to‐noise ratio >4), with a correlation coefficient of 0.998. When the curve was derived from a mixture of GPS molecular species, only a slight variation in the ion intensity ratio of GPS 18:0–18:1 to GPS di 16:0 occurred. The method provides the possibility of quantifying GPS 18:0–18:1 among GPS species in the low nanogram range.