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Sequence‐informative fragmentation of peptides up to a molecular weight of 4.6 kDa in plasma‐desorption mass spectrometry
Author(s) -
Vorst H. J.,
van Tilborg M. W. E. M.,
van Veelen P. A.,
Tjaden U. R.,
van der Greef J.
Publication year - 1990
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290040607
Subject(s) - chemistry , fragmentation (computing) , mass spectrometry , peptide , desorption , sequence (biology) , chromatography , molecular mass , biochemistry , organic chemistry , adsorption , enzyme , computer science , operating system
The potential of plasma‐desorption mass spectrometry (PDMS) in peptide sequencing is investigated. This paper shows that PDMS spectra recorded for longer times and using larger amounts of peptide than used for obtaining molecular weight information, provides sequence information on peptides of up to 4.5 kDa molecular weight. This approach strongly enhances the utility of PDMS for validation of the sequence of recombinant peptides.