Premium
Peptide sequencing with electrospray ionization on a magnetic sector mass spectrometer
Author(s) -
Meng C. K.,
McEwen C. N.,
Larsen B. S.
Publication year - 1990
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290040505
Subject(s) - chemistry , fragmentation (computing) , ion , electrospray , electrospray ionization , tandem mass spectrometry , mass spectrometry , analytical chemistry (journal) , collision induced dissociation , ionization , ion source , chromatography , protein mass spectrometry , organic chemistry , computer science , operating system
Sequence information is obtained by in‐source fragmentation of electrospray‐generated peptide ions. The complete sequences of leucine enkephalin, angiotensin I, and renin substrate are determined from their fragment ions and consume only low picomole amounts of material. The method involves collision‐induced fragmentation of singly and multiply charged molecular ions in the electrospray ion source and does not involve tandem mass spectrometric instrumentation. The charge on the fragment ions is determined by the spacing of the isotope peaks.