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Fragment pathway analysis using automated tandem mass spectrometry on an ion‐trap mass spectrometer
Author(s) -
Pannell L. K.,
Quanlong Pu,
Mason R. T.,
Fales H. M.
Publication year - 1990
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290040403
Subject(s) - chemistry , ion trap , mass spectrometry , tandem mass spectrometry , fragmentation (computing) , tandem , top down proteomics , hybrid mass spectrometer , selected reaction monitoring , analytical chemistry (journal) , quadrupole ion trap , spectrometer , chromatography , computer science , aerospace engineering , physics , engineering , operating system , quantum mechanics
A “key‐sequence” procedure is presented for the automated tandem mass spectrometric analysis of compounds on a Finnigan ion‐trap mass spectrometer. This allows fragmentation pathways of a range of masses or even a complete spectrum to be prepared automatically, obviating the tiresome preparation and optimization of individual scan‐editor files. The procedure is limited by the speed of the driving computer; an “IBM‐AT”, for example, permits more than 10 mass units to be scanned per minute. It is calibrated with perfluorotributylamine and methyl stearate is used to demonstrate its results. The Finnigan ion‐trap “programming option” is necessary for implementation of the procedure.