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Collisionally activated dissociation of peptides using a quadrupole ion‐trap mass spectrometer
Author(s) -
Kaiser Raymond E.,
Cooks R. Graham,
Syka John E. P.,
Stafford George C.
Publication year - 1990
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290040109
Subject(s) - chemistry , ion trap , mass spectrometry , tandem mass spectrometry , quadrupole ion trap , dissociation (chemistry) , triple quadrupole mass spectrometer , hybrid mass spectrometer , tandem , ion , top down proteomics , collision induced dissociation , analytical chemistry (journal) , ionization , quadrupole mass analyzer , mass spectrum , selected reaction monitoring , chromatography , materials science , organic chemistry , composite material
The ion‐trap mass spectrometer can be used to record mass and multi‐stage tandem mass spectra (MS n ) on small peptides. The compounds are ionized by Cs + surface ionization, injected into the trap, mass selected, activated by collision, resulting in dissociation and mass‐selectively ejected. Tandem mass spectrometric data on sub‐fmol amounts of Gramicidin S are shown, along with tandem mass spectrometric and MS n data on two other peptides. The experiment is distinguished by its very high sensitivity and the high quality of sequence information obtained.