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Methodological approach to the characterization of diacylphosphatidyl cholines in rabbit lung lavage fluid by fast‐atom bombardment mass spectrometry
Author(s) -
Allmaier G.,
Schmid E. R.,
Gasser H.,
Strohmaier W.,
Schlag G.
Publication year - 1990
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290040107
Subject(s) - chemistry , fast atom bombardment , chromatography , mass spectrometry , high performance liquid chromatography , ion , analytical chemistry (journal) , organic chemistry
Strong evidence exists that the distribution of phospholipids in the lung is a function of the degree of adult respiratory distress syndrome. The capabilities of positive‐ion fast‐atom bombardment (FAB) mass spectrometry for the determination of the relative distribution of intact diacylphosphatidylcholine species in lung lavage fluid were investigated. Two different FAB matrices and two different isolation/purification procedures—extraction/thin layer chromatography—high performance liquid chromatography (TLC‐HPLC)—have been evaluated. In addition the relative fatty acid composition of the diacylphosphatidylcholines was determined by negative‐ion FAB mass spectrometry using the [RCOO] − fragment ion. These results were compared with those obtained by gas chromatographic determination of the fatty acid methylesters.