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A coordinated approach towards the molecular weight determination of peptides by gel electrophoresis and fast‐atom bombardment mass spectrometry
Author(s) -
Camilleri Patrick,
Haskins Neville J,
Hill Andrew J,
New Anthony P,
Games D. E.
Publication year - 1989
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290031209
Subject(s) - chemistry , fast atom bombardment , mass spectrometry , chromatography , molecular mass , protein mass spectrometry , neurotensin , capillary electrophoresis , tandem mass spectrometry , biochemistry , enzyme , receptor , neuropeptide
The molecular weight of peptides can be determined by fast‐atom bombardment mass spectrometry (FAB‐MS) following polyacrylamide gel electrophoresis (PAGE). Initial results combining the fast and efficient separation of peptides by PAGE and the unambiguous determination of molecular weights by FAB‐MS have been demonstrated for the three peptides bradykinin, neurotensin and gramicidin S. This method has also been applied to the determination of the molecular weights of two fragments from the tryptic digest of horse‐heart cytochrome c .