Premium
Cinnamic acid derivatives as matrices for ultraviolet laser desorption mass spectrometry of proteins
Author(s) -
Beavis Ronald C.,
Chait Brain T.,
Fales H. M.
Publication year - 1989
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290031207
Subject(s) - chemistry , cinnamic acid , mass spectrometry , ultraviolet , chromatography , desorption , organic chemistry , adsorption , physics , quantum mechanics
The paper reports the discovery of three new matrices for the matrix‐assisted laser desorption of proteins. These new matrices (sinapic, ferulic and caffeic acids) are cinnamic acid derivatives that have several pratical advantages over the nicotinic acid matrices previously used. These materials form much less intense photochemically generated adduct peaks in the protein quasimolecular ion signal and the adduct peaks that are present are easier to resolve. These new matrices are produce intense protonated‐molecule ions from all of the proteins (over 50) so far examined. These new matrices are also very stable in a vacuum, allowing for their convenient use in very high vacuum applications (e.g., Fourier transform ion cyclotron resonance mass spectrometry).