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Translational energy release and stereochemistry of steroids. 14. Epimeric dihydroxy steroids of the androstane series
Author(s) -
Zaretskii Z. V. I.,
Ghosh D.,
Brenton A. G.,
Bey J. H.
Publication year - 1989
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290031002
Subject(s) - chemistry , metastability , kinetic energy , dehydration , androstane , ion , mass spectrometry , stereochemistry , mass spectrum , organic chemistry , chromatography , biochemistry , quantum mechanics , physics
The stereochemistry of dihydroxy steroids, both the mode of the A/B ring junctions and the configuration of OH groups, may be determined from translational energy (T 50% ) measurements for the loss of a CH 3 radical, from the ratios of metastable‐ion peak heights to those of the main beam (determined for the dehydration reactions), and by comparing unimolecular and collision‐induced, mass‐analysed ion kinetic energy spectra of the new main beam of [M H 2 O] + ˙ ions (i.e. those formed via dehydration of metastable molecular ions of epimeric hydroxy steroids in the first field‐free region of a double‐focusing mass spectrometer).