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Observation of sequence‐specific peptide fragmentation using extended tandem mass spectrometry experiments
Author(s) -
Schey Kevin L.,
Schwartz Jae C.,
Cooks R. Graham,
Caprioli R. M.
Publication year - 1989
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290030910
Subject(s) - chemistry , tandem mass spectrometry , mass spectrometry , fragmentation (computing) , tandem , mass spectrum , selected reaction monitoring , protonation , protein mass spectrometry , molecule , analytical chemistry (journal) , isobaric labeling , peptide , ion , chromatography , organic chemistry , biochemistry , materials science , computer science , composite material , operating system
Reaction‐intermediate spectra are reported for peptide mixtures using a hybrid BEQQ and a pentaquadrupole mass spectrometer. In this extended tandem mass spectrometry scan, the protonated molecule and a terminal fragment ion are selected with the first and third mass analyzers, respectively. A subsequent linked scan at constant B/E (hybrid instrument) or a scan of the middle quadrupole (pentaquadrupole instrument) produces the reaction‐intermediate spectrum. The resulting spectra are simpler than conventional tandem mass spectrometry daughter‐ion spectra and display only sequence ions of one group, viz. A, B and C or X, Y and Z. This information facilitates structural elucidation and is obtainable on nanomole amounts of peptide.