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Laser‐induced desorption of proteins
Author(s) -
Salehpour M.,
Perera I.,
Kjellberg J.,
Hedin A.,
Islamian M. A.,
Håkansson P.,
Sundqvist B. U. R.
Publication year - 1989
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290030804
Subject(s) - chemistry , mass spectrometry , laser , desorption , excimer laser , analytical chemistry (journal) , ion , range (aeronautics) , optics , chromatography , adsorption , physics , materials science , composite material , organic chemistry
Laser‐induced desorption mass spectrometry has been applied to a number of proteins in the mass range 5000–150 000 u. The beam from an excimer‐laser‐pumped dye‐laser at 266 nm has been focused to a spot of about 50 μm in diameter with irradiances in the 10 7 W/cm 2 region. A linear time‐of‐flight mass spectrometer has been used for mass spectrometric measurements, where positive and negative secondary ions of large proteins have been studied. The effect of different experimental parameters on the protein ion‐signal intensities are discussed.