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Negative‐ion mass spectrometry of substituted adenine bases and adenosine nucleosides
Author(s) -
Hocart Charles H.,
Schlunegger Urs P.
Publication year - 1989
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290030802
Subject(s) - chemistry , ion , mass spectrum , mass spectrometry , chemical ionization , dissociation (chemistry) , electron ionization , ionization , trimethylsilyl , analytical chemistry (journal) , spectral line , base (topology) , kinetic energy , medicinal chemistry , organic chemistry , chromatography , mathematical analysis , physics , mathematics , quantum mechanics , astronomy
The negative‐ion chemical ionization (ammonia, 5 Pa source pressure) mass spectra of a series of substituted adenine bases, adenosine nucleosides, and the trimethylsilyl derivatives of the nucleosides are described. Selected ions from these spectra were subject to collisionally activated dissociation with mass‐analysed ion kinetic energy (CAD/MIKE) analysis of the products and the spectra assessed for information content. In addition to observing strong peaks due to quasimolecular ions and heterocyclic‐base ions, it proved possible to differentiate between 2′‐, 3′‐ and 5′‐deoxy and between 2′‐ and 3′‐ O ‐methyl isomers. The negative‐ion chemical ionization spectra of four methyladenines are essentially identical, but could be clearly distinguished from each other by CAD/MIKE analysis.