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Strategy for sequencing peptides as mono‐ and dilithiated adducts using a hybrid tandem mass spectrometer
Author(s) -
Leary Julie A.,
Williams Todd D.,
Bott Geoffrey
Publication year - 1989
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290030608
Subject(s) - chemistry , adduct , protonation , ion , dissociation (chemistry) , tandem mass spectrometry , mass spectrometry , tandem , lithium (medication) , medicinal chemistry , organic chemistry , chromatography , materials science , composite material , medicine , endocrinology
A strategy for sequencing peptides using the mono‐ and dilithiated quasimolecular ions is proposed. Low energy collision‐induced dissociation (CID) of the monolithiated species produced primarily [A n +LiH] + and [B n +H+OLi] + ions while that of the dilithiated species produced [A n +LiH] + and [Y n +Li+H] + ions. The precursor parent ion in the monolithiated adduct is proposed to be a mixture of lithiated neutral peptide (1) and protonated lithium salt (2). Mechanisms for the formation of the [A n +LiH] + and [Y n +Li+H] + ions are proposed. Four peptides of known composition but unknown sequence were successfully sequenced using the combined data from the CID spectra of the mono‐ and dilithiated species.