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Sequence determination of multiply charged peptide molecular ions by electrospray–ionization tandem mass spectrometry
Author(s) -
Barinaga Charles J.,
Edmonds Charles G.,
Udseth Harold R.,
Smith Richard D.
Publication year - 1989
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290030512
Subject(s) - chemistry , tandem mass spectrometry , protein mass spectrometry , electrospray ionization , mass spectrometry , melittin , top down proteomics , tandem , ion , electrospray , chromatography , extractive electrospray ionization , sample preparation in mass spectrometry , quadrupole mass analyzer , ionization , analytical chemistry (journal) , peptide , organic chemistry , biochemistry , materials science , composite material
Electrospray ionization generates multiply charged peptide molecular ions which are efficiently dissociated by collision with a neutral gas to provide sequence‐specific daughter ions. The scope for application of this phenomenon is illustrated by the nearly complete sequencing of melittin (relative molecular mass ( M r ) 2846.5) employing a tandem quadrupole mass spectrometer having a mass limit of m / z 1400.