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Determination of lovastatin acid in serum by gas chromatography/mass spectrometry
Author(s) -
WangIverson David,
Ivashkiv Eugene,
Jemal Mohammed,
Cohen Allen I.
Publication year - 1989
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290030504
Subject(s) - chemistry , bstfa , derivatization , chromatography , chemical ionization , mass spectrometry , gas chromatography–mass spectrometry , gas chromatography , trimethylsilyl , detection limit , selected ion monitoring , reagent , ion , ionization , organic chemistry
The acid form of lovastatin, an HMG‐CoA reductase inhibitor, was analyzed by gas chromatography/negativeion chemical ionization mass spectrometry after derivatization with pentafluorobenzyl bromide and bis‐(trimethylsilyl)trifluoroacetamide (BSTFA). Mass spectrometry of this derivative produced a dominant [M–181] − ion under chemical ionization conditions using ammonia as the reagent gas. The limit of detection was approximately 2 pg injected on column.