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Linked scans of peptides and protein digests: Amino acid sequence determination of components of complex mixtures
Author(s) -
Morris Howard R.,
Chatterjee Ashraf,
Panico Maria,
Green Brian N.,
da Silva Maria A. A. Almeida,
Hartley Brian S.
Publication year - 1989
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290030406
Subject(s) - chemistry , biopolymer , sequence (biology) , amino acid , peptide sequence , biochemistry , nucleotide , chromatography , posttranslational modification , enzyme , computational biology , organic chemistry , gene , polymer , biology
Two‐sector linked‐scan analysis of an unpurified proteolytic digest of a pyruvate decarboxylase enzyme (60 000 Da) has allowed the discovery and assignment of an amino‐terminal post‐translational modification and processing event. A difference in amino acid sequence from that predicted by a recently published nucleotide sequence has also been found. These results illustrate both the use and considerable potential of linked‐scan methods for the analysis of complex biopolymer mixtures.