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Comparison of amino acid‐sequence analysis by electron ionization and negative‐ion chemical ionization mass spectrometry
Author(s) -
Reinhard H.,
Lauber R.,
Schlunegger U. P.
Publication year - 1989
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290030402
Subject(s) - chemistry , chemical ionization , electron ionization , ion , ionization , mass spectrometry , mass spectrum , ion source , atmospheric pressure laser ionization , analytical chemistry (journal) , thermal ionization mass spectrometry , tripeptide , ambient ionization , thermal ionization , chromatography , amino acid , organic chemistry , biochemistry
The positive electron ionization and negative chemical ionization mass spectra of 15 different derivatives of the tripeptide Phe‐Ala‐Leu have been compared. Total ion currents and ion currents of sequence‐characterizing ions have been measured and compared. The negative‐ion spectra, using 10% carbon dioxide in argon as moderator gas, proved to be simpler and contained more abundant sequence ions than the positive electron ionization spectra.