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Capillary‐zone electrophoresis/fast‐atom bombardment mass spectrometry: Design of an on‐line coaxial continuous‐flow interface
Author(s) -
Moseley M. Arthur,
Deterding Leesa J.,
Tomer Kenneth B.,
Jorgenson James W.
Publication year - 1989
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1290030310
Subject(s) - chemistry , capillary electrophoresis , mass spectrometry , analyte , analytical chemistry (journal) , fast atom bombardment , capillary action , chromatography , capillary electrophoresis–mass spectrometry , electrophoresis , coaxial , tandem , tandem mass spectrometry , electrospray ionization , materials science , electrical engineering , composite material , engineering
An on‐line coaxial continuous‐flow capillary‐zone electrophoresis/fast‐atom bombardment mass spectrometry (CZE/FAB‐MS) interface is described. This interface is shown to be capable of acquiring mass spectra in an online fashion from low femtomole amounts of peptides while maintaining high (hundreds of thousands of plates) electrophoretic separation efficiencies. Active electrophoretic transport of the analytes directly to the FAB probe tip obviates the need for a transfer line from the end of the CZE capillary to this point, and thereby precludes the zone broadening that would otherwise occur both within such a transfer line and in the connections between the CZE column and the transfer line. The capability of acquiring an on‐line tandem mass spectrometry (MS/MS) spectrum of an electrophoretically separated analyte using this interface is also demonstrated.