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Dynamic calibration and dissolved gas analysis using membrane inlet mass spectrometry for the quantification of cell respiration
Author(s) -
Yang Tae Hoon,
Wittmann Christoph,
Heinzle Elmar
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1251
Subject(s) - chemistry , mass spectrometry , corynebacterium glutamicum , analytical chemistry (journal) , carbon dioxide , respiration , calibration , inlet , chromatography , mechanical engineering , biochemistry , botany , statistics , mathematics , organic chemistry , biology , engineering , gene
A membrane inlet mass spectrometer connected to a miniaturized reactor was applied for dynamic dissolved gas analysis. Cell samples were taken from 7 mL shake flask cultures of Corynebacterium glutamicum ATCC 13032, and transferred to the 12 mL miniaturized reactor. There, oxygen uptake and carbon dioxide and its mass isotopomer production rates were determined using a new experimental procedure and applying nonlinear model equations. A novel dynamic method for the calibration of the membrane inlet mass spectrometer using first‐order dynamics was developed. To derive total dissolved concentration of all carbon dioxide species ( C T ) from dissolved carbon dioxide concentration ([CO 2 ] aq ), the ratio of C T to [CO 2 ] aq was determined by nonlinear parameter estimation, whereas the mass transfer coefficient of CO 2 was determined by the Wilke–Chang correlation. Subsequently, the suitability of the model equations for respiration measurements was examined using residual analysis and the Jarque–Bera hypothesis test. The resulting residuals were found to be random with normal distribution, which proved the adequacy of the application of the model for cell respiration analysis. Hence, dynamic changes in respiration activities could be accurately analyzed using membrane inlet mass spectrometry with the novel calibration method. Copyright © 2003 John Wiley & Sons, Ltd.