Use of liquid chromatography/time‐of‐flight mass spectrometry and multivariate statistical analysis shows promise for the detection of drug metabolites in biological fluids | Zendy

Plumb Robert S. | Zendy; Stumpf Chris L. | Zendy; Granger Jennifer H. | Zendy; CastroPerez Jose | Zendy; Haselden John N. | Zendy; Dear Gordon J. | Zendy

Premium

Use of liquid chromatography/time‐of‐flight mass spectrometry and multivariate statistical analysis shows promise for the detection of drug metabolites in biological fluids

Author(s) -

Plumb Robert S.,

Stumpf Chris L.,

Granger Jennifer H.,

CastroPerez Jose,

Haselden John N.,

Dear Gordon J.

Publication year - 2003

Publication title -

rapid communications in mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 136

eISSN - 1097-0231

pISSN - 0951-4198

DOI - 10.1002/rcm.1250

Subject(s) - chemistry , chromatography , mass spectrometry , metabolite , liquid chromatography–mass spectrometry , metabolomics , metabolite profiling , tandem mass spectrometry , biological fluids , time of flight mass spectrometry , biochemistry , ion , organic chemistry , ionization

The process of metabolite identification is essential to the drug discovery and development process; this is usually achieved by liquid chromatography/tandem mass spectrometry (LC/MS/MS) or a combination of liquid chromatography/mass spectrometry (LC/MS) and nuclear magnetic resonance (NMR) spectroscopy. Metabolite identification is, however, a time‐consuming process requiring an experienced skilled scientist. Multivariate statistical analysis has been used in the field of metabonomics to elucidate differences in endogenous biological profiling due to a toxic effect or a disease state. In this paper we show how a combination of liquid chromatography/time‐of‐flight mass spectrometry (LC/TOFMS) and multivariate statistical analysis can be used to detect drug metabolites in a biological fluid with no prior knowledge of the compound administered. Copyright © 2003 John Wiley & Sons, Ltd.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research